Morris Water Maze Online

Morris Water Maze Online

The protocol, step by step

Here's the classic recipe. Labs tweak it, but if you're new to the maze, start here.

  1. Set up: the pool, opaque water at a constant temperature, a hidden platform and distal cues around the room.
  2. Handle the animals for a few days before testing begins.
  3. Acquisition: 4 trials a day for 4 to 6 days from varied start points, up to 60 to 90 s per trial. Gently guide any animal that doesn't find the platform.
  4. Platform rest: 10 to 30 s on the platform after each trial, time to take in the view.
  5. Probe: remove the platform and allow a 60 s swim, usually 24 h after the last training trial.
  6. Controls: a visible-platform session to check vision and motivation.
  7. Optional: reversal learning, with the platform moved.

The standard reference is Vorhees & Williams (2006).

Further reading